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dnaE [2017-12-07 13:27:48]
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dnaE [2017-12-07 13:27:48]

lagging strand DNA polymerase III (alpha subunit), part of the replisome
locus
BSU29230
pI
6.02
mw
125.14 kDa
protein length
1115 aa Sequence Blast
gene length
3345 bp Sequence Blast
function
DNA replication (lagging strand)
product
lagging strand DNA polymerase III (alpha subunit)
essential
yes
ec
2.7.7.7
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    2,991,269 → 2,994,616

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • required for bacteriophage SPP1 replication [Pubmed|23268446]

    • Protein family

  • DnaE subfamily (according to Swiss-Prot)

    • Structure

  • [PDB|3E0D] (from ''Thermus aquaticus'', 39% identity) [Pubmed|18691598]

    • [SW|Localization]

  • forms foci, the foci dissappear after the formation of DNA mismatches in a [protein|E2A8B04CD729332F9E6A13195181983F751F2F25|MutS]-dependent manner or after DNA replication arrest [Pubmed|21958350]
  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|65B5EA4DBDF5B260DED754B8A7B226AA88F17B55|dnaE]-[gene|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [PubMed|14593098,16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

    • regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed]
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    References

    Reviews

  • 22933559,28073967

    • Original publications

  • 11721055,14593098,16267290,20122408,23563155,23017159,21958350,23268446,24062730,24106089,18691598,28575448,28878042